WST-5
Flammability | 1 | |
Toxicity | 2 | |
Body Contact | 2 | |
Reactivity | 0 | |
Chronic | 0 | |
SCALE: Min/Nil=0 Low=1 Moderate=2 High=3 Extreme=4 |
Intermediate. Novel tetrazolium compound for use in colourimetric assays determining the
number of viable cells in proliferation, in cytotoxicity chemosensitivity assays.
Bioreduced by cells into a water- soluble formazan by dehydrogenases found in
metabolically active cells. The WST range of reagents are readily reduced by NADP or NADP,
in the presence of an electron coupling reagent to produce yellow or purple formazans.
C52-H44-N12-Na2-O16-S6, "2, 2'-dibenzothiazolyl-5, 5'-bis[4-di(2-
sulfoethyl)carbamoylphenyl]-3, 3'-(3, 3'-dimethoxy-4, 4'-biphenylene)ditetrazolium,
disodium salt", "2, 2'-dibenzothiazolyl-5, 5'-bis[4-di(2-sulfoethyl)carbamoylphenyl]-3,
3'-(3, 3'-dimethoxy-4, 4'-biphenylene)ditetrazolium, disodium salt", WST5, "tetrazolium
formazan generator"
Harmful by inhalation, in contact with skin and if swallowed.
Irritating to eyes and respiratory system.
Accidental ingestion of the material may be harmful; animal experiments indicate that ingestion of less than 150 gram may be fatal or may produce serious damage to the health of the individual. Harmful if swallowed. Considered an unlikely route of entry in commercial/industrial environments.
This material can cause eye irritation and damage in some persons. Irritating to eyes.
Skin contact with the material may be harmful; systemic effects may resultfollowing absorption. The material is not thought to be a skin irritant (as classified using animal models). Temporary discomfort, however, may result from prolonged dermal exposures. Good hygiene practice requires that exposure be kept to a minimum and that suitable gloves be used in an occupational setting. Harmful in contact with skin.
If inhaled, this material can irritate the throat andlungs of some persons. Harmful by inhalation. Irritating to respiratory system. Persons with impaired respiratory function, airway diseases and conditions such as emphysema or chronic bronchitis, may incur further disability if excessive concentrations of particulate are inhaled.
Principal routes of exposure are usually by skin contact/absorption and inhalation of generated dust. No human exposure data available. For this reason health effects described are based on experience with chemically related materials. As with any chemical product, contact with unprotected bare skin; inhalation of vapor, mist or dust in work place atmosphere; or ingestion in any form, should be avoided by observing good occupational work practice.